To estimate the total number of the sexual lineage cells, the following formula was applied: Sperm analysis Epididymal sperms were extracted from the cut edge of the caudal epididymis and were placed in a watch glass filled with 5 mL normal saline solution [41, 42]
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Clin Geriatr Med 38:565591 Trabado S, Al-Salameh A, Croixmarie V, Masson P, Corruble E, Feve B, Colle R, Ripoll L, Walther B, Boursier-Neyret C, Werner E, Becquemont L, Chanson P (2017) The human plasma-metabolome: reference values in 800 french healthy volunteers