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Following this, the samples were sequentially incubated with primary and secondary antibodies, and nuclei were stained with 4',6-diamino-2-phenylindole (DAPI) (Beyotime Biotechnology, China)
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Strikingly, addition of 50 M compound 3 , 6 , 9 or 10 for 24 h reduced GST-PFO staining intensity by 2.32.8-fold in U18666A-treated SH-SY5Y cells, respectively
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