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Drugmakers had 60 days to meet the administration's demands voluntarily, or, the letters stated, "if you refuse to step up, we will deploy every tool in our arsenal to protect American families from continued abusive drug pricing practices." Then came the closed-door negotiations, which included the threat of tariffs stemming from an investigation into whether pharmaceutical imports posed a threat to national security

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Raised the temperature of the reaction mixture to 25-30C and stirred for 2 hr at the same temperature