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FDA Category: C (C = Studies in laboratory animals have revealed adverse effects on the fetus (teratogenic, embryocidal, etc.) but there are no controlled studies in pregnant women

Lipofectamine 2000 (Invitrogen, Carlsbad, USA) was used to deliver plasmid constructs (2.2 g/6.0-cm Petri dish) into cells in accordance with the manufacturers instructions

In addition, this criterion is based on the symptoms but not the cause, and based on what patients report in their questionnaires

Sullivan, S

Measurements of islet hormone secretion Mouse islets were used acutely, except for studies with PTX, wherein islets were treated overnight with the toxin