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Commercially available enzyme-linked immunosorbent assays (ELISA) were used for determination of total GLP-1 (736 and 936) (ALPCO, Salem, NH), GLP-2 (133 and 333) (Merck Millipore, Burlington, MA) and DPP4 (R&D Systems, Minneapolis, MN) plasma concentrations and were carried out according to the manufacturers instructions

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to study the effect of Amuc_1434 protein, also purified as a His-tag protein from overproducing E.coli , on colorectal cancer LS174T cells