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Women with obesity and/or diabetes (the population using GLP-1 medications) already face higher pregnancy complication rates even without medication

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The purified genomic DNA served as the template and was amplified by PCR (98 C for 30 sec, 25 (98 C for 10 s, 55 C for 20 s, 72 C for 20 s), 72 C for 5 min) with the forward primer S-D-Bact-0341-b-S-17 (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCW-GCAG-3) and reverse primer S-D-Bact-0785-a-A-21 (5-GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3) with Illumina adapters attached

This is entirely separate from Mounjaro's T2D coverage

Group 3: CRE-AgNPs treated (negative control): This group was treated with 50 mg/kg body weight of CRE-AgNPs dissolved in distilled water for 7 consecutive days once daily