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The reaction mixture was stirred at room temperature for 12 h

More water means a lower concentration (more dilute), which means you draw a larger volume per dose

The cells treated with Sp showed slight enhancement in levels of GSH (~1.2 fold), together with minor alterations in GSSG levels, compared to untreated samples which exhibited higher GSSG content, thus indicating efficient recycling of GSSG to GSH (Fig

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