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Importantly, iodoacetamide (IAA, a known thiol-reactive compound) was not able to oxidize roGFP when cells were exposed to non-lethal or subtoxic concentrations of this compound, indicating that an electrophilic attack on roGFP is not sufficient to cause a spectral change consistent with its oxidation (Supplementary Fig

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The cumulative impact of these mechanisms is diagrammed in Figure 1, highlighting the systemic nature of ROS-induced reproductive failure

These reactions typically become less pronounced with each subsequent injection as the tissue adapts