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For the non-targeted metabolite analysis, the tissue metabolites were extracted in 80% MeOH at a final tissue concentration of 50 mg/mL for 24 h at 80 C

Operators typically use chromatography to achieve this stepmost commonly reverse-phase high-performance liquid chromatography (RP-HPLC) or ion exchange (IEX)

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The advantages of such systems are the simplicity of implementation, low costs, and the possibility to assess the transendothelial electrical resistance (TEER) rather easily as a parameter characterizing modeled barrier integrity