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Afterwards, spatial transcriptomics data were normalized using the SCTransform function

Indeed, catabolism of mitochondrial GSH supplies cysteine for ironsulfur cluster synthesis 23

Potential identities were then added to an inhouse structure library including.mol files from Chemspider (Royal Society of Chemistry, London, United Kingdom) and then targeted on the initial raw data according to the following parameters: a 5 ppm mass tolerance, a 2 mDa mass tolerance for predicted structure fragments, a 5% CCS deviation, and multiple adducts (+H, + Na, +K, + Li, +NH4) within 5 ppm and 0.1 minutes

and pyrimidine metabolism (Additional file 12: Figure S12)

That usually happens around the 4 to 6 week mark with consistent use