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The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)
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Bioinformatics analysis 16S-Amplicon processing pipeline The 16S-amplicon processing pipeline was modified from 16S Bacteria/Archaea SOP v1 of Microbiome Helper workflows [31]
Kuchakulla M, Masterson T, Arora H, Kulandavelu S, Ramasamy R
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