To investigate the function of CBS in this process, we silenced its expression by CRISPR/cas9 mediated gene knockout strategy (sg CBS ) in SCD erythroblasts (Fig
Seeds were sown on half-strength Murashige and Skoog (MS) medium (Duchefa) containing 1% sucrose (Sigma-Aldrich)
PubMed Ng, F.M., Sun, J., Sharma, L., Libinaki, R., Jiang, W.J., & Gianello, R
Like a delicate piece of machinery, its effectiveness is entirely dependent on its three-dimensional structure remaining intact
DEG were identified based on the following thresholds: padj 0.05 for comparison A and padj 0.01 for comparisons B and C, with absolute fold-change values 1.25