There are two methods to follow: Select a consistent date for ring changes, irrespective of the month's length
Those included various baseline characteristic including demographics (age, gender, race), comorbidities (e.g., liver diseases, chronic lower respiratory diseases, diabetic complications, obesity and neoplasm), medications (e.g., insulin, metformin, angiotensin-converting enzyme inhibitors (ACEIs), angiotensin II receptor blockers (ARBs), tacrolimus, cyclosporine, mycophenolate mofetil (MMF), and corticosteroids), as well as laboratory tests and physical findings (e.g., hemoglobin, glycated hemoglobin (HbA1c) levels, lipid profile, estimated glomerular filtration rate (eGFR) and systolic blood pressure (SBP))
Avoid -80C, which provides no additional benefit and complicates retrieval
Markers did not differ between horses co-infected by two or three strains and those infected by a single strain ( t -test, P 0.22)
This helps maintain and increase lean muscle mass, delay age-related muscle loss, reduce post-exercise soreness, and accelerate recovery, providing multiple supports for muscle growth and functional maintenance