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Mechanistically, HGF inhibits inflammatory M1-like macrophage production of pro-inflammatory cytokines and chemokines (such as IL-1, IL-6, TNF, CXCL1, CXCL10, and CCL2) by disrupting nuclear factor NF-B signaling (Giannopoulou et al., 2008) through the enhancement of the heme oxygenase-1 (HO-1) transcriptional pathway and the inactivation of the GSK-3 pathway (Kamimoto et al., 2009a,b

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Keeping the reconstituted mixtures strictly refrigerated between 2C and 8C halts molecular degradation and prevents any potential drop in the bacteriostatic efficacy of the diluent, guaranteeing that long-term studies yield high-fidelity data