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Hematoxylin-eosin staining Testes, epididymides, and ovaries were fixed in 4% paraformaldehyde overnight at room temperature, dehydrated through a graded ethanol series (50%, 60%, 70%, 80%, 95%, and 100%
High IV dosage or high local concentrations of methylene blue may cause formation of methemoglobin and cyanosis
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Diestrus was defined by the presence of small leukocytes, and proestrus was defined by the presence of clumps of large, round, nucleated epithelial cells
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