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mRNA was then isolated from the tissues using E.Z.N.A

Approximately 1000 PGCs per sample were transferred into a 1.5-mL tube containing 500 L of wash buffer

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After transfection of the sgRNA libraries into CAG-C9-3 cells, we obtained two stable cell populations carrying constitutively expressed Cas9 and sgRNAs (Trim-library-1 and Trim-library-2)

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