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Due to the increased lipid load in vehicle-treated HFD + L-NAME mice, KEGG pathway analysis identified increased protein levels in cholesterol and lipid metabolism as well as redox signaling and lipid homeostasis in peroxisome in LV of HFD + L-NAME compared to control mice (Fig

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For activity screening, we adapted a GSTO1 substrate assay that relies on the quantification of the reduction of S -(4-nitrophenacyl)glutathione (4-NPG), a GSTO1-specific substrate 17 , using recombinant human GSTO1 in a high-throughput screening format

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