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You may not get enough B12 in your diet if you are: Vegan Vegetarian Over 50 Living with digestive conditions that affect nutrient absorption, such as IBS, celiac, or Crohns disease Taking certain medications like metformin or proton pump inhibitors (PPIs) What are the symptoms of B12 deficiency

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Any switch should happen under medical supervision with a clear dosing plan

For immunoblot, tissues were homogenized in RIPA buffer (EMD Millipore) with protease and phosphatase inhibitor cocktail (BioTools)