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Here is a simple frequency guide based on your hair loss stage: Early-stage thinning or preventive use - once daily application is sufficient Moderate hair fall or visible thinning - twice daily application, morning and night Post-hair transplant recovery - follow your DHI doctor's specific protocol for frequency and timing Maintenance after achieving desired results - once daily to sustain density and prevent regression Dos and Don'ts of Hair Serum Application Dos: Do apply to a clean scalp for maximum absorption Do use consistently - skipping days significantly slows results Do massage gently to boost scalp circulation Do store the serum away from direct sunlight and heat to preserve active ingredients Do take before-and-after photographs every 4 weeks to track progress objectively Do use alongside DHI's broader hair care protocol for synergistic results Don'ts Don't apply to dirty or product-laden hair - always cleanse first Don't use on broken, irritated, or infected scalp skin Don't rinse the serum out - it is a leave-on treatment Don't apply more than the recommended dose - more is not more effective Don't mix with other scalp treatments without consulting your DHI specialist Don't expect overnight results - hair growth is a biological process that takes time Hair Growth Serum for Men vs Women: Is There a Difference