Metabolic adaptations from the stack are not permanent
Think of this preparation phase as creating the optimal internal environment for the peptide to work effectively
When your stomach is empty, it's a much less chaotic environment
The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord
doi:10.1371/journal.pone.0103982 Chiang KC, Sun CC, Chen MH, Huang CY, Hsu JT, Yeh TS, Chen LW, Kuo SF, Juang HH, Takano M et al (2016) MART-10, the new brand of 1alpha,25(OH)2D3 analog, is a potent anti-angiogenic agent in vivo and in vitro