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They rarely describe failure states: persistent cloudiness from aggregated peptide, gel-like consistency from incomplete dissolution of a large pellet, or a precipitate forming hours after initial clarity due to slow aggregation

In this study, iPSCs have been differentiated using DMEM/F12 medium supplemented with L-glutamin, heparin, and B27 for astrocytes or vasculife basal medium supplemented with FGF, acid ascorbic, hydrocortisone, glutamine, and growth factor for brain endothelial cells