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Due to the complex interaction of different factors that contribute to an effective immune response, there may be more than one correlate of protection, and the mechanisms of protection during natural infection may differ from those seen after vaccination
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This depends on the individual pain tolerance, needle size, injection technique, and injection site
Besides, reactive glial cells, members of the NVU, are likely to contribute to the permeability of the BBB observed in cerebral hypoxia through downregulation of paracellular proteins such as Claudin-5 (CLDN5), occludin, and ZO-1 (Obermeier et al., 2013)
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