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Once cells were proliferating, they were moved up to higher cell volumes and adapted to DMEM cell culture media with 20% dFBS, 50 U ml 1 penicillin and streptomycin and 1 mM sodium pyruvate for at least 1 week before experimental setup

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Compounds that target mutant p53 are loosely divided into broad-spectrum compounds that target diverse p53 mutants and allele-specific compounds that target a single mutant or a different group of similar mutants (Table 2 )