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Neuronal nitric oxide synthase is a novel biomarker for the interstitial cells of cajal in stress-induced diarrhea-dominant irritable bowel syndrome
The biofilm biomass attached to the wells was then stained with 200 L 0.05% (w/v) CV and incubated a further 1 h at 37C, 150 rpm
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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration These practices support consistent experimental outcomes and reduce variability across assays
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