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On ice, cells were scraped and collected in extraction buffer containing methanol:acetonitrile:H 2 O (4:4:2), 15 M glutaric acid and 0.5% formic acid

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Increasing BMI has been correlated with higher mortality risks, including a 29% rise in overall mortality and over a 200% increase in diabetes-related mortality for every 5-unit BMI increase above 25 kg/m (4)

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