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TRAP-sequencing (along with sequencing of matched inputs where appropriate) was performed by Cambridge Genomic Services, utilising the Clonetech - SMART-Seq v4 Ultra Low input RNA library preparation, along with sequencing on the Illumina Nextseq 500 platform
GLP-1 receptor activation GLP-1 receptor activation was determined using an in vitro bioluminescence resonance energy transfer (BRET)-based assay that measures changes in intracellular cyclic-AMP levels
Neither is approved for human use by the FDA or any other major regulatory body, and the findings below come from laboratory and animal studies
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By bridging traditional practices with modern scientific understanding, this review underlines the growing relevance of shiitake not only as a functional food and therapeutic resource but also as a component of sustainable food systems and bio-based waste management strategies
