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Eluate incubated with TEV protease was dialysed against 25 mM Tris (pH 7.5), 150 mM NaCl and 1 mM DTT overnight at 4 C, then incubated with Ni-NTA resin for 1 h to remove the His-tagged protease
Methods Enzymol 328: 575-592
The skincare forums are dotted with similar stories - people who tried copper peptides expecting firmer, younger-looking skin and ended up with the opposite
SAHF detection Cells were fixed with 4% paraformaldehyde and washed with PBS
10.1016/j.jddst.2015.12.008 152 LiH
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