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Nystrm T, Gutniak MK, Zhang Q, Zhang F, Holst JJ, Ahrn B, et al
A 10 l qPCR reaction was amplified in duplicate wells for each sample 4.5 l of cDNA mixture (100 ng cDNA template + rest nuclease-free water) with 5 l iTaq Universal SYBR Green Supermix, and 0.5 l forward and reverse primer mixtures (10 pm)
Final Checks and Follow-up: When the study is finished, participants have their last health checks