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During caloric restriction, whole-body EAA requirements increase, meaning the body needs more of these amino acids at the exact time that appetite suppression makes them harder to consume from food

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Metabolic profiling, enhanced by PCA and orthogonal partial least squares discriminant analysis (OPLS-DA), demonstrated a clear metabolomic divergence between the WT and GR mutant strains (Figure 5A), indicating the significant impact of GR knockout on the metabolic framework

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