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This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Young Scientists (B) Grant Number JP15K19036 (SH), JSPS Grant-in-Aid for Young Scientists Grant Number JP19K16271 (SH), Japan Science and Technology Agency (JST) ACT-X Grant Number JPMJAX201A (SH), RIKEN FY2018 Incentive Research Projects (SH), and Naito Grant for female scientists after maternity leave (SH)

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Cystine/glutamate antiporter solute carrier family 7 member 11 (SLC7A11) is a major transporter regulating cysteine in tumor cells and is induced in response to a variety of stimuli, such as oxidative stress and electrophilic compounds ( xCT transcription, which enhance the GSH levels and protect tumor cells against oxidative stress ( + T cells in the tumor stromal region attenuated platinum resistance through STAT1 phosphorylation and xCT downregulation in fibroblasts ( Figure 2 ) ( Figure 2 GSH peroxidase 4 (GPX4) The tripeptide GSH consists of glutamate, cysteine, and glycine and serves as a cysteine storage and transport form ( via chaperone-mediated autophagy ( via glutathione depletion and ROS accumulation ( Hydrogen sulfide H 2 S is produced in mammalian cells by three enzymes, including cystathionine -synthase (CBS), cystathionine -lyase (CSE), and 3-mercaptopyruvate sulfurtansferase (3MST) in mammalian cells

Progress Tracking Tracking weekly is the most reliable way to assess whether a protocol is working, particularly given the delayed response timeline