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This three-phase randomized controlled clinical trial involves 1961 overweight or obese adults (none of them suffering from diabetes, average weight 105 kg, average body mass index 38 kg/m2), the clinical trial was conducted at 129 sites in 16 countries in Asia, Europe, and North and South America
The cells were isolated by centrifugation and allowed to recover in 5 mL of YPD broth at 30 C in a rotary shaker for 5 h prior to selective growth
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