2.6 Microbial bioinformatics analysis Microbial bioinformatics analysis was performed as previously described ( 2.7 Preparation of serum samples for mass spectrometry analysis Serum samples were thawed on ice, and 100 L of each sample was mixed with 300 L of precooled methanol and acetonitrile (2:1, v/v)
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The significance of iron buildup in inflamed tissue, resulting in cellular toxicity, in autoimmune and autoinflammatory illnesses associated with arthritis, such as OA, remains ambiguous [35]
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