After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)
Insert the needle at a slight angle through the stopper
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This process removes water and makes the peptide a stable, lightweight powder, perfect for shipping and long-term storage
( d ) GSEA analysis of data from ( b, c ) run against metabolic gene sets (defined in 10 )