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In the study conducted by Secchi et al

Recently, through combined application with the CRISPR-Cas9-based base editor, this approach allowed us to characterize individual residues critical for the function of DND1 that is an essential gene in PGC development, 22 in which, a CAG promoter showed to have a high mutagenesis efficiency in vivo

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The protein concentration in the solution of each microcentrifuge tube was then determined by NanoDrop ONE, and the protein-containing solution was collected

J Plast Reconstr Aesthet Surg 63:11861195