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Protein isolation and western blotting For standard western blotting, tissues were lysed in RIPA buffer and extracted protein content was determined using the BCA protein assay (Sigma, St

PRECAUTIONS: Vitamin B12 deficiency that is allowed to progress for longer than 3 months may produce permanent degenerative lesions of the spinal cord

GHK-Cu is incorporated into an increasing variety of cosmetic products, primarily serums, anti-aging creams and post-procedure care

Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors

Skin cells take about 28 to 40 days to turn over