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Most relevant for this study, gain-of-function experiments, using ectopic overexpression of wildtype or constitutively active Rab mutants (Rab4, 7, 8, 9) or a Rab7 guanine nucleotide exchange factor, reduced cholesterol accumulation in NPC1 mutant cells and patient fibroblasts [12, 15, 16] (reviewed in [72])