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Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later

N-Hydroxysuccinimide (NHS) Conjugation Some ADCs utilize N-hydroxysuccinimide (NHS) esters to react with lysine (Lys) residues on the antibody, forming stable amide bonds (Figure 9)

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