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Multidrug resistance-related transport proteins in isolated human brain microvessels and in cells cultured from these isolates

a , Scheme of the in vitro sgRNA competition assay performed in HEK-293T SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with a pool of 5 control sgRNAs (sgControl, gray) and sgRNAs targeting TXNRD2 (pink) and SLC25A40 (green) (left)

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The resulting buffy coat was collected, resuspended in PBS, and centrifuged again (200 x g, 8 min, 20C, Universal 320, Hettich, Tuttlingen, Germany)