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One potential mechanism is that H 2 S 2 and H 2 S 3 produced by 3MST readily react with free cysteine and GSH to produce Cys-SSH and GSSH, and react with cysteine residues of proteins to generate persulfurated proteins (Figs 2 and 8a)

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The GSH/GSSG-Glo Assay is a luminescent assay that can quantify total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells

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