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Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures

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Since vitamin B 12 is found predominantly in foods of animal origin, strict vegetarian and vegan diets can result in vitamin B 12 deficiency (2, 16)

[3] : 313 The most common cause in developed countries is impaired absorption due to loss of gastric intrinsic factor (IF), required for absorption

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