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Histopathology Complete histopathology was performed in 24-mo-old female [Old Con ( n = 16), and Old mAb ( n = 16)] and male mice [Old Con ( n = 15), and Old mAb ( n = 17)] following 6 mo of mAb treatment, as well as in female mice at death from the longevity study [Old Con ( n = 30), and Old mAb ( n = 20)]

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Parallel cultures diluted 1:6 in PBS were incubated at 37 C with shaking for 3 h, then treated with 1% glucose, 5 mM sodium pyruvate and 0.5% casamino acids, or vehicle for 30 min

217 PatrizioA.SpechtC

A patient suffering from severe, treatment-refractory hyperadrenergic POTSexperiencing debilitating tachycardia, tremors, and massive adrenaline surgeswas found to have a genetic polymorphism in their COMT pathway