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Animal studies do not always capture subtle or long-term adverse effects that might manifest in humans

For protocols requiring 250 mcg or less, 3 mL works well since it keeps doses in the 5-10 unit range where accuracy is high

doi:10.1073/pnas.80.3.707

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In a model of lipopolysaccharide (LPS)-induced visceral hypersensitivity and repeated water avoidance stress (WAS) in rats, liraglutide effectively reduced visceral allodynia by suppressing pro-inflammatory cytokine production and improving colonic barrier integrity [59]