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Primarily for reconstituting lyophilized peptides and other research compounds requiring multiple sterile draws over time
The cells were then treated in triplicate with 2012, 2020 (negative control), and Gambogic Acid (positive control) at titrating concentrations ranging from 100 M to 200 nM and allowed to incubate for 45 min in a 37 C incubator with 5% CO 2
Optimal dosage ensures steady melanin reduction without stressing the body
For histology, mouse livers were fixed in 4% paraformaldehyde in PBS at 4C for 24 h and then switched to 70% ethanol
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