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For the total and active GLP-1 assay, dried pellets were resuspended in RIA buffer, and for MS analysis, dried pellets were resuspended in PBS, and in each case, the protein concentration was determined using the Pierce BCA Protein Assay Kit (Prod# 23225, ThermoFisher Scientific, France)

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Weeks three to six, you're progressively increasing activity and starting physical therapy

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