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Improvements in firmness and tone usually become visible between 4 and 8 weeks

Unreconstituted lyophilised powders remain stable at room temperature for brief periods during transport, but long-term storage demands dedicated refrigeration between 2 and 8 degrees Celsius or freezing at minus 20 degrees Celsius

Using a lesser diluent, like simple sterile water, is possible for single-use applications, but it offers no protection against contamination once the vial's septum has been punctured

Volume = 500 / 2,500 = 0.20 mL

The most common experimental dose in animal studies (Sikiric et al.) is 10 g/kg/day intraperitoneally or orally in rats