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After centrifugation, cell pellets were then collected and resuspended with stimulation buffer [HBSS supplemented with 5 mM HEPES, 0.5 mM IBMX and 0.1% (w/v) BSA, pH 7.4] to a density of 0.6 million cells per mL and added to 384-well culture plates (3,000 cells per well)

The outcomes were normalized employing the fluorescence intensity of 0.1 mg/mL quinine sulfate in 0.1 M H 2 SO 4 as a reference 38