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International Journal of Obesity and Related Metabolic Disorders

For patients experiencing moderate to severe symptoms, doses may be increased to 400-500 mcg daily

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2.5 Sample preparation After evaluation of seizure behavior, the blood samples were collected from the hearts of all rats under deep anesthesia using 80 mg/kg ketamine with 10 mg/kg xylazine (Kitazawa et al., 2005

All samples were labeled with N -(2-aminoethyl) rhodamine 6G-amide bis(trifluoroacetate) (R6G) by using the EDC/NHS coupling reaction with a weight ratio of polypeptide to R6G at 20:1 (Fig