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In mouse studies, measurable effects on organ function appeared within 10-14 days of treatment initiation

This sequential approach serves two purposes: first, to eliminate immortal time bias by properly aligning treatment initiation with follow-up start (T0) and, second, to maximize statistical power by allowing individuals to contribute data across multiple trials

For example, mice fed a high-fat diet for 21 days have both lipid and protein compositional changes in cellular plasma membranes that result in an impaired capacity for oxidative phosphorylation, increased reactive oxygen species (ROS) production, hepatosteatosis and insulin resistance (Shama et al., 2023)

The intensity of this tingling increases with dosage size

Turkish J Med Sci 44(3):471475