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Determination of single-strand DNA (ssDNA) breaks, mitochondrial membrane depolarization, caspase cleavage and DNA fragmentation Cells were seeded, pretreated with BSO (400 M) for 24 h followed by treatment with L-PAM (30 M)

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The cupric reducing antioxidant capacity (CUPRAC) assay quantifies antioxidant activity through the reduction of Cu 2+ -neocuproine to Cu + -neocuproine under near-neutral conditions (pH 7.0)

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