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The peptide peak(s) were eluted with a gradient starting at 95% Buffer A, and 5% Buffer B, and changing linearly to 94.6% A and 5.4% B at 1 min, then linearly to 68% A and 32% B at 16 min, then linearly to 20% A and 80% B at 18 min, where it was held isocratically until 24 min

Common side effects can include pain or redness at the injection site, mild diarrhea, itching, or a feeling of swelling all over the body

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Although classic DIA analysis with an experiment specific spectral library is superior to dDIA, it is much easier to implement the dDIA workflow making it easier accessible to the general proteomics community

Several hypotheses are being explored: Neuroplasticity effects: Chronic activation of amylin pathways may strengthen neuronal networks that favor satiety over hunger